CELL INCORPORATION STUDY OF [99mTc]Tc-NAB-PACLITAXEL ON HUMAN COLORECTAL ADENOCARCINOMA CELL LINE

Objective: The development of new radiopharmaceuticals capable specifically imaging cancer is an important research area. For this reason, the aim study to investigate cell binding novel developed nanoradiopharmaceutical ([99mTc]Tc-nab-PTX) on HT-29 line (human colorectal adenocarcinoma cells).
 Material and Method: In study, nab-PTX was labeled with [99mTc]Tc, labeling efficiency in vitro stability were investigated by radioactive thin layer chromatography (RTLC). Then, incorporation [99mTc]Tc-nab-PTX performed using line.
 Result Discussion: According obtained results, high radiochemical purity (>99%). found be stable saline for 6-h, medium up 2-h. It determined that had a greater activity cells than R/H-[99mTc]NaTcO4 at 30 min. results promising future vivo studies [99mTc]Tc-nab-PTX.